IL-12 Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 85.9 kDa and the accession number is P43431&P43432.
生物活性 | 1. Measured by its ability to mouse IL12RB2-His in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 15-90 ng/mL. |
产品描述 | IL-12 Protein, Mouse, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 85.9 kDa and the accession number is P43431&P43432. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His, C-hFc |
蛋白编号 | P43431&P43432 |
别名 | Il-12b, Il12p40, interleukin 12A, Ll12a, Il-12p40, p35, 白细胞介素, p40, IL-12p35, Il-12a, 白介素 |
蛋白构建 | The p35 subunit of Mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The p40 subunit of Mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with the Fc region of Human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified. |
蛋白纯度 | > 85 % as determined by SDS-PAGE |
分子量 | 85.9 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
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IL-12 Protein, Mouse, Recombinant (His & hFc) Il-12b Il12p40 interleukin 12A Ll12a Il-12p40 p35 白细胞介素 p40 IL-12p35 Il-12a 白介素 recombinant recombinant-proteins proteins protein