Powder: -20°C for 3 years | In solvent: -80°C for 1 year
ATN-224 (Bis(choline)tetrathiomolybdate) 抑制内皮细胞中的 SOD1 活性,这种作用呈剂量依赖性,IC50 为 17.5±3.7 nM。 ATN-224 是一种口服 Cu2+/Zn2+-超氧化物歧化酶 1 (SOD1) 抑制剂。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 266 | 现货 | ||
2 mg | ¥ 378 | 现货 | ||
5 mg | ¥ 579 | 现货 | ||
10 mg | ¥ 928 | 现货 | ||
25 mg | ¥ 1,790 | 现货 | ||
50 mg | ¥ 2,980 | 现货 | ||
100 mg | ¥ 4,770 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 637 | 现货 |
产品描述 | ATN-224 (Bis(choline)tetrathiomolybdate) inhibits SOD1 activity in endothelial cells, an effect that is dose dependent with an IC50 of 17.5±3.7 nM. ATN-224 is an oral Cu2+/Zn2+-superoxide dismutase 1 (SOD1) inhibitor. |
靶点活性 | SOD1 (endothelial cells):17.5±3.7 nM |
体外活性 | ATN-224 is an orally-available inorganic small molecule that inhibits the copper/zinc-dependent enzyme, superoxide dismutase 1 (Cu/Zn-SOD1), in endothelial and tumor cells[2] amd it is able to inhibit SOD1 activity in endothelial cells, an effect that is dose dependent with an IC50 of 17.5±3.7 nM. ATN-224 inhibits FGF-2-induced ERK1/2 phosphorylation in a dose-dependent and time-dependent manner with an IC50 between 1.25 and 2.5 μM, consistent with the IC50 for the inhibition of proliferation[1], and it inhibits the proliferation of both HUVEC (IC50=1.4±0.3 μM; n=5) and it is also able to inhibit the activity of purified bovine SOD1 with an IC50 of 0.33±0.03 μM after 24 hours of incubation. ATN-224 has a specific and high affinity for copper ions (108 mol/L-1) and shows no binding to calcium, iron, magnesium, zinc, or manganese ions at concentrations up to 1 mM as determined by isothermal titration calorimetry. The SOD1 inhibition by ATN-224 is time dependent, reaching maximal inhibition at ~16 hours. ATN-224 seems to inhibit SOD1 by depleting the enzyme of copper. |
体内活性 | Inhibition of angiogenesis when ATN-224 is given by oral gavage occurred before there is measurable depletion of copper in either plasma or copper from the Matrigel plug. ATN-224 also significantly (P<0.05) inhibits angiogenesis in the Matrigel plug model in mice either when added directly to the plug or when given by oral gavage. This result shows that ATN-224 inhibits angiogenesis independently of copper depletion[1]. |
别名 | 四硫钼酸二胆碱, Bis(choline)tetrathiomolybdate |
分子量 | 432.56 |
分子式 | C10H28MoN2O2S4 |
CAS No. | 649749-10-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (115.59 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.3118 mL | 11.5591 mL | 23.1182 mL | 57.7955 mL |
5 mM | 0.4624 mL | 2.3118 mL | 4.6236 mL | 11.5591 mL | |
10 mM | 0.2312 mL | 1.1559 mL | 2.3118 mL | 5.7795 mL | |
20 mM | 0.1156 mL | 0.578 mL | 1.1559 mL | 2.8898 mL | |
50 mM | 0.0462 mL | 0.2312 mL | 0.4624 mL | 1.1559 mL | |
100 mM | 0.0231 mL | 0.1156 mL | 0.2312 mL | 0.578 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
ATN-224 649749-10-0 Others 四硫钼酸二胆碱 ATN224 ATN 224 Bis(choline)tetrathiomolybdate Inhibitor inhibitor inhibit