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IL-12 Protein, Mouse, Recombinant (His)

IL-12 Protein, Mouse, Recombinant (His)

产品编号 TMPY-02350
别名: Il-12b, p35, IL-12p35, Ll12a, Il-12a, interleukin 12A, Il12p40, 白细胞介素, Il-12p40, 白介素, p40

IL-12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is P43431&P43432.

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IL-12 Protein, Mouse, Recombinant (His)
规格 价格/CNY 货期 数量
50 μg ¥ 3,430 现货
100 μg ¥ 5,480 5日内发货
200 μg ¥ 8,730 5日内发货
500 μg ¥ 16,130 5日内发货
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千万补贴 助力科研
BCA蛋白浓度测定试剂盒限时半价
产品目录号及名称: IL-12 Protein, Mouse, Recombinant (His) (TMPY-02350)
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生物活性
技术参数
产品性质
生物活性 1. Measured by its ability to bind biotinylated recombinant mouse IL12RB2 in a functional ELISA. 2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-10 ng/mL.

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产品描述 IL-12 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.3 kDa and the accession number is P43431&P43432.
种属 Mouse
表达系统 HEK293 Cells
标签 C-His
蛋白编号 P43431&P43432
别名 Il-12b, p35, IL-12p35, Ll12a, Il-12a, interleukin 12A, Il12p40, 白细胞介素, Il-12p40, 白介素, p40
蛋白构建 The p35 subunit of Mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The p40 subunit of Mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
蛋白纯度 > 90 % as determined by SDS-PAGE

TargetMol

分子量 60.3 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

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Keywords

IL-12 Protein, Mouse, Recombinant (His) Il-12b p35 IL-12p35 Ll12a Il-12a interleukin 12A Il12p40 白细胞介素 Il-12p40 白介素 p40 recombinant recombinant-proteins proteins protein

 

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