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Human respiratory syncytial virus (RSV) Fusion Protein (aa 1-526, His)

产品编号 TMPY-05687

别名: F Protein, HRSVgp8

Human respiratory syncytial virus (RSV) Fusion Protein (aa 1-526, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.04 kDa and the accession number is A0A023RA53.

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Human respiratory syncytial virus (RSV) Fusion Protein (aa 1-526, His)

规格	价格/CNY		货期	数量
100 μg	￥ 6,390		5日内发货

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生物活性	Testing in progress
产品描述	Human respiratory syncytial virus (RSV) Fusion Protein (aa 1-526, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.04 kDa and the accession number is A0A023RA53.

种属	RSV
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	A0A023RA53
别名	F Protein, HRSVgp8
蛋白构建	The Human respiRatory syncytial virus (RSV) Fusion (AHX57537.1) (Met1-Met526) was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	> 90% as determined by SDS-PAGE.
分子量	57.04 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, pH 7.5, 300 mM NaCl, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.

参考文献

1. Martin-Gallardo A. et al., 1993, J Gen Virol. 74 : 453-8. 2. Jose AM. et al.,1997, J Gen Virol. 78: 2411-8. 3. Feldman SA. et al., 1999, J Virol. 73: 6610-7. 4. García-Beato R. et al., 2000, J Gen Virol. 81: 919-27. 5. Zlateva KT. et al., 2004, J Virol. 78: 4675-83. 6. Trento A. et al., 2006, J Virol. 80: 975-84.

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Keywords

Human respiratory syncytial virus (RSV) Fusion Protein (aa 1-526, His) F Protein HRSVgp8 recombinant recombinant-proteins proteins protein

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