Human respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G Protein (93% Homology) (His)

Human respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G Protein (93% Homology) (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 26.3 kDa and the accession number is P27022-1.

规格	价格/CNY		货期	数量
100 μg	￥ 6,390	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 6,390

5日内发货

生物活性	Testing in progress
产品描述	Human respiratory syncytial virus (RSV) (A, rsb1734) glycoprotein G/RSV-G Protein (93% Homology) (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 26.3 kDa and the accession number is P27022-1.

种属	RSV
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	P27022-1
别名	G Protein
蛋白构建	The glycoprotein G extracellular domain (Asn 66-Arg297) of Human respiRatory syncytial virus A (93% homologous with strain rsb1734) (P27022-1) was expressed, with a C-terminal polyhistidine tag.
蛋白纯度	> 92 % as determined by SDS-PAGE
分子量	26.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl, pH 8.0, 10% glycerol, 0.5 mM EDTA, 0.5 mM PMSF. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. HRSV G protein is a type II glycoprotein of 289-299 amino acids (depending on the virus strain) with a signal/anchor hydrophobic domain and is extensively modified by the addition of both N-and O-linked oligosaccharides to achieve the mature form of 8-9 kDa. The C-terminal ectodomain of the G protein has a central region and four cysteines which are conserved in all HRSV isolates and have been proposed as the putative receptor binding site. The G protein mediates attachment of the virus to the host cell membrane by interacting with heparan sulfate, initiating the infection. As similar to mucins in amino acid compositions, the RSV G protein can interact with host CX3CR1, the receptor for the CX3C chemokine fractalkine, and thus modulates the immune response and facilitate infection. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and hemagglutinating activities.