规格

价格

库存

数量

5 μg

¥ 257

现货

10 μg

¥ 393

现货

20 μg

¥ 648

现货

50 μg

¥ 1,250

现货

100 μg

¥ 2,100

现货

生物活性

产品描述	IL-6R Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 65.3 kDa and the accession number is P08887.
生物活性	1.Measured by its ability to enhance the activity of IL6 on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 30-125 ng/mL in the presence of 100 ng/mL of recombinant human IL6. 2.Loaded Recombinant Human IL-6R Protein, hFc Tag on ProA Biosensor, can bind Recombinant Human IL-6 Protein, no Tag with an affinity constant of 0.51µM as determined in BLI assay (Routinely tested). 3. Immobilized Recombinant Human IL6 / Interleukin-6 Protein at 2μg/mL (100μL/well) can bind Recombinant Human IL-6R / CD126 Protein (ECD, Fc Tag) , the EC50 is 5-15 ng/mL.
研究背景	Interleukin 6 receptor (IL-6R) also known as CD126 (Cluster of Differentiation 126) is a type I cytokine receptor. The low concentration of a soluble form of IL-6 receptor (SIL-6R) acts as an agonist of IL-6 activity. In the IL-6R/CD126/IL6R system, both a membrane-bound IL-6R and a SIL-6R protein can mediate IL-6 signals into the cells through the interaction of gp130. The resulting IL-6/sIL-6R protein complex is also capable of binding to gp130 and inducing intracellular signaling. Through this so-called 'trans-signaling' mechanism, IL-6 can stimulate cells that lack an endogenous mIL-6R. High levels of IL-6 and SIL-6R have been reported in several chronic inflammatory and autoimmune diseases as well as in cancer.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy
种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	P08887
蛋白构建	A DNA sequence encoding the human IL6R (NP_000556.1) (Met1-Pro365) was expressed with the Fc region of human IgG1 at the C-terminus. Predicted N terminal: Leu 20
蛋白纯度	> 90% as determined by SDS-PAGE.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名	interleukin 6 receptor, IL6RQ, IL-6RA, IL6RA, IL-6R-1, IL-6R, IL6Q, gp80, CD126
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	65.3 kDa (predicted); 70-100 kDa (reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

IL-6R alpha/CD126 Protein, Human, Recombinant (hFc)

IL-6R alpha/CD126 Protein, Human, Recombinant (hFc)

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