IL-12 Protein, Human, Recombinant (His)

IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2.

规格	价格/CNY		货期	数量
50 μg	￥ 2,940	5日内发货
500 μg	￥ 19,100	5日内发货

规格

价格/CNY

货期

数量

50 μg

￥ 2,940

5日内发货

500 μg

￥ 19,100

5日内发货

生物活性	Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is typically 0.04-0.4 ng/mL.
产品描述	IL-12 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60.13 kDa and the accession number is P29459-1&NP_002178.2.

种属	Human
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P29459-1&NP_002178.2
别名	IL12, NKSF1, IMD28, CLMF2, 白细胞介素, NKSF2, NKSF, CLMF, IMD29, IL-12B, IL12 p70, NFSK, P35, 白介素, interleukin 12A, IL-12A
蛋白构建	The p35 subunit of Human IL-12, termed as IL12A (P29459-1) (Met1-Ser219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The p40 subunit of Human IL-12, termed as IL12B (NP_002178.2) (Met1-Ser328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
分子量	60.13 kDa (predicted)

内毒素	< 5 EU/mg of the protein.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.

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IL-12 Protein, Human, Recombinant (His)

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