CD47 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 17.1 kDa and the accession number is Q61735-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 3,820 | 5日内发货 | ||
100 μg | ¥ 8,840 | 5日内发货 |
生物活性 | Immobilized Recombinant Mouse CD47 Protein (His & AVI Tag), Biotinylated on streptavidin precoated (2 μg/mL,100 μL/well) can bind Recombinant Mouse SIRP alpha / CD172a Protein (Fc Tag), the EC50 is 14-52 ng/mL. |
产品描述 | CD47 Protein, Mouse, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 17.1 kDa and the accession number is Q61735-1. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His-Avi |
蛋白编号 | Q61735-1 |
别名 | B430305P08Rik, AA407862, IAP, Itgp, CD47 molecule, AI848868, AW108519, 9130415E20Rik |
蛋白构建 | The Mouse CD47 (Q61735-1)(Met1-Lys140) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. |
蛋白纯度 | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
分子量 | 17.1 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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CD47 Protein, Mouse, Recombinant (His & Avi), Biotinylated B430305P08Rik AA407862 IAP AW 108519 Itgp CD47 molecule AI848868 AA 407862 AW-108519 AW108519 9130415E20Rik AA-407862 AI-848868 AI 848868 recombinant recombinant-proteins proteins protein