Powder: -20°C for 3 years | In solvent: -80°C for 1 year
M-31850 (1H-Benz[de]isoquinoline-1,3(2H)-dione, 2,2'-(iminodi-2,1-ethanediyl)bis-) 是一种竞争性的 β-己糖胺酶选择性抑制剂,能够抑制人HexA (IC50:6.0 µM) 及HexB (IC50:3.1 µM)。它还能够竞争性抑制 β-N-acetyl-D-hexosaminidaseOfHex2 (Ki:2.5 µM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 255 | 现货 | ||
10 mg | ¥ 392 | 现货 | ||
25 mg | ¥ 756 | 现货 | ||
50 mg | ¥ 947 | 现货 | ||
100 mg | ¥ 1,566 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 376 | 现货 |
产品描述 | M-31850 (1H-Benz[de]isoquinoline-1,3(2H)-dione, 2,2'-(iminodi-2,1-ethanediyl)bis-) is a potent, selective and competitive β-hexosaminidase (Hex) inhibitor(human HexA and human HexB with IC50s of 6.0 μM and 3.1 μM, respectively). IT also competitively inhibits β-N-acetyl-D-hexosaminidase OfHex2 with a Ki of 2.5 μM. |
靶点活性 | HexB (human):3.1 µM (IC50), HexA (human):6.0 µM (IC50), OfHex2:2.5 μM (Ki) |
体外活性 | M-31850 increases the half-life of the mutant Hex A from Adult forms of Tay-Sachs (ATSD) cells more than two-fold at 44° C, relative to the enzyme heated in the presence of DMSO. M-31850 acts as a classic competitive inhibitor of Hex (Km increases and Vmax is unaffected by increasing amounts of M-31850), with a Ki of 0.8 μM.M-31850 shows some activity towards Jack Bean Hex (JBHex) and bacterial Hex from Streptomyces plicatus (SpHex) (IC50 of 280 M and >500 M for JBHex and SpHex, respectively)[1] |
别名 | 1H-Benz[de]isoquinoline-1,3(2H)-dione, 2,2'-(iminodi-2,1-ethanediyl)bis- |
分子量 | 463.48 |
分子式 | C28H21N3O4 |
CAS No. | 281224-40-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 5 mg/mL (10.79 mM), when pH is adjusted to 6 with HCl. Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.1576 mL | 10.788 mL | 21.5759 mL | 53.9398 mL |
5 mM | 0.4315 mL | 2.1576 mL | 4.3152 mL | 10.788 mL | |
10 mM | 0.2158 mL | 1.0788 mL | 2.1576 mL | 5.394 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
M-31850 281224-40-6 Others inhibit M31850 OfHex2 β-hexosaminidase non-carbohydrate Inhibitor M 31850 1H-Benz[de]isoquinoline-1,3(2H)-dione, 2,2'-(iminodi-2,1-ethanediyl)bis- inhibitor