SOST Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 1,950 | 现货 | ||
100 μg | ¥ 3,430 | 5日内发货 | ||
200 μg | ¥ 6,060 | 5日内发货 | ||
500 μg | ¥ 12,830 | 5日内发货 |
生物活性 | Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10 T1/2 cells. The ED50 for this effect is typically 0.2-1 μg/mL. |
产品描述 | SOST Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.5 kDa and the accession number is Q9BQB4-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-His |
蛋白编号 | Q9BQB4-1 |
别名 | VBCH, sclerostin, DAND6, CDD, SOST1 |
蛋白构建 | The mature form of Human SOST (NP_079513.1) (Gln24-Tyr213) was fused with a polyhistidine tag at the N-terminus. |
蛋白纯度 |
> 90 % as determined by SDS-PAGE
|
分子量 | 22.5 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Sclerostin, the protein product of the SOST gene, is a potent inhibitor of bone formation. Sclerostin protein is widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteeoblasts differentiated for 21 days, and was originally identified as an important regulator of bone remodeling, homeostasis, and links bone resorption and bone apposition. Recent studies have revealed that Sclerostin protein inhibits the bone growth probably by binding to the extracellular domain of the Wnt coreceptors LRP5 and LRP6 and disrupting Wnt-induced Frizzled-LRP complex formation. |
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SOST Protein, Human, Recombinant (His) DAND-6 DAND 6 VBCH sclerostin DAND6 CDD SOST 1 SOST1 SOST-1 recombinant recombinant-proteins proteins protein