The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (SAPK2a, CSBP), p38 beta (SAPK2b), p38 delta (SAPK4), and p38 gamma (SAPK3/ERK6). p38 alpha and p38 beta are widely expressed p38 isoforms that are involved in the regulation of cell proliferation, differentiation, development, and stress response. p38 delta, also known as MAPK13, is a regulator of differentiation-dependent gene expression in keratinocytes and has been a regulator of surface epithelial differentiation and apoptosis. p38 delta protein is upregulated in Cholangiocarcinoma (CC) relative to hepatocellular carcinoma (HCC) and normal biliary tract tissues. p38 delta is important for motility and invasion of CC cells, suggesting that p38 delta may play an important role in CC metastasis. p38 delta is expressed in the epidermis, suggesting a role for p38 delta in regulating differentiation. p38 delta is the major p38 isoform driving suprabasal involucrin gene expression and that p38 delta directly regulates ERK1/2 activity via the formation of a p38 delta-ERK1/2 complex. Recent emerging evidence suggests that the p38 stress MAPK pathway may function as a tumor suppressor through regulating Ras-dependent and -independent proliferation, transformation, invasion, and cell death by isoform-specific mechanisms. p38 delta has an important role in promoting cell proliferation and tumor development in the epidermis and may have therapeutic implications for skin cancer.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 μg | ¥ 3,170 | 5日内发货 |
生物活性 | The specific activity was determined to be >30 nmol/min/mg using MBP as substrate. |
产品描述 | The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (SAPK2a, CSBP), p38 beta (SAPK2b), p38 delta (SAPK4), and p38 gamma (SAPK3/ERK6). p38 alpha and p38 beta are widely expressed p38 isoforms that are involved in the regulation of cell proliferation, differentiation, development, and stress response. p38 delta, also known as MAPK13, is a regulator of differentiation-dependent gene expression in keratinocytes and has been a regulator of surface epithelial differentiation and apoptosis. p38 delta protein is upregulated in Cholangiocarcinoma (CC) relative to hepatocellular carcinoma (HCC) and normal biliary tract tissues. p38 delta is important for motility and invasion of CC cells, suggesting that p38 delta may play an important role in CC metastasis. p38 delta is expressed in the epidermis, suggesting a role for p38 delta in regulating differentiation. p38 delta is the major p38 isoform driving suprabasal involucrin gene expression and that p38 delta directly regulates ERK1/2 activity via the formation of a p38 delta-ERK1/2 complex. Recent emerging evidence suggests that the p38 stress MAPK pathway may function as a tumor suppressor through regulating Ras-dependent and -independent proliferation, transformation, invasion, and cell death by isoform-specific mechanisms. p38 delta has an important role in promoting cell proliferation and tumor development in the epidermis and may have therapeutic implications for skin cancer. |
种属 | Human |
表达系统 | Baculovirus-Insect Cells |
标签 | GST |
蛋白编号 | O15264 |
别名 | P38 δ/MAPK13, MAPK13, PRKM13, MAPK-13, p38δ, p38delta, mitogen-activated protein kinase 13, SAPK4 |
蛋白构建 | A DNA sequence encoding the full length of human MAPK13 (O15264) (Met1-Leu365) was fused with the GST tag at the N-terminus. Activated in vitro by MAP2K6 (10422-H20B1). |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 68.4 kDa (predicted) |
内毒素 | < 1.0 EU per μg of the protein as determined by the LAL method |
缓冲液 | Supplied as sterile 50 mM Tris-HCl, 150 mM NaCl, 0. 25 mM DTT, 0. 1 mM EDTA, 0. 1 mM PMSF, 25 % glycerol, pH 7.5. Please contact us for any concerns or special requirements. Please refer to the specific buffer information in the hard copy of CoA. |
复溶方法 | A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information. |
存储 |
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products. |
研究背景 | The p38 family of mitogen-activated protein kinases (MAPK) includes p38 alpha (SAPK2a, CSBP), p38 beta (SAPK2b), p38 delta (SAPK4), and p38 gamma (SAPK3/ERK6). p38 alpha and p38 beta are widely expressed p38 isoforms that are involved in the regulation of cell proliferation, differentiation, development, and stress response. p38 delta, also known as MAPK13, is a regulator of differentiation-dependent gene expression in keratinocytes and has been a regulator of surface epithelial differentiation and apoptosis. p38 delta protein is upregulated in Cholangiocarcinoma (CC) relative to hepatocellular carcinoma (HCC) and normal biliary tract tissues. p38 delta is important for motility and invasion of CC cells, suggesting that p38 delta may play an important role in CC metastasis. p38 delta is expressed in the epidermis, suggesting a role for p38 delta in regulating differentiation. p38 delta is the major p38 isoform driving suprabasal involucrin gene expression and that p38 delta directly regulates ERK1/2 activity via the formation of a p38 delta-ERK1/2 complex. Recent emerging evidence suggests that the p38 stress MAPK pathway may function as a tumor suppressor through regulating Ras-dependent and -independent proliferation, transformation, invasion, and cell death by isoform-specific mechanisms. p38 delta has an important role in promoting cell proliferation and tumor development in the epidermis and may have therapeutic implications for skin cancer. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
p38 delta/MAPK13 Protein, Human, Recombinant (GST), Activated in vitro MAPK 13 P38 δ/MAPK13 MAPK13 PRKM13 SAPK-4 SAPK 4 MAPK-13 PRKM-13 p38δ p38delta mitogen-activated protein kinase 13 SAPK4 PRKM 13 recombinant recombinant-proteins proteins protein