CSF3R/G-CSFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 68 kDa and the accession number is Q99062-1.
规格 | 价格/CNY | 货期 | 数量 | |
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100 μg | ¥ 3,070 | 现货 | ||
200 μg | ¥ 5,420 | 5日内发货 | ||
500 μg | ¥ 11,490 | 5日内发货 |
生物活性 |
Measured by its ability to inhibit the GCSF-induced proliferation of NFS60 mouse myeloid cells. The ED50 for this ettect is typically 0.5-2ug/mL in the presence of 0.125ng/mL of recombinant human GCSF.
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产品描述 | CSF3R/G-CSFR Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 68 kDa and the accession number is Q99062-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | Q99062-1 |
别名 | CSF3R, GCSFR, colony stimulating factor 3 receptor (granulocyte), G-CSF R, CD114 |
蛋白构建 | The extracellular domain (Met 1-Pro 621) of Human G-CSF receptor (NP_000751.1) precursor was fused with a polyhistidine tag at the C-terminus. |
蛋白纯度 |
> 85 % as determined by SDS-PAGE
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分子量 | 68 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Granulocyte Colony Stimulating Factor Receptor (G-CSFR), also known as CD114, which belongs to the cytokine receptor superfamily, is a cell surface receptor for colony stimulating factor 3 (CSF3). It is a critical regulator of granulopoiesis. This type I membrane protein has a composite structure consisting of an immunoglobulin(Ig)-like domain, a cytokine receptor-homologous (CRH) domain and three fibronectin type III (FNIII) domains in the extracellular region. Mutations in the G-CSF receptor leading to carboxy-terminal truncation transduce hyperproliferative growth responses, and are implicated in the pathological progression of severe congenital neutropenia (SCN) to acute myelogenous leukemia (AML). Additionally, autocrine/paracrine stimulation of G-CSFR may be important in the biology of solid tumors, including metastasis. |
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CSF3R/G-CSFR Protein, Human, Recombinant (His) granulocyte CSF3R GCSFR CD 114 colony stimulating factor 3 receptor (granulocyte) CD-114 colony stimulating factor 3 receptor G-CSF R CD114 recombinant recombinant-proteins proteins protein