CD40 Ligand Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 17.7 kDa and the accession number is P29965.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,170 | 5日内发货 | ||
1 mg | ¥ 22,400 | 5日内发货 |
生物活性 | Immobilized Human CD40 Ligand (His Tag) at 2 μg/mL (100 μL/well) can bind Human CD40 (ECD, hFc Tag), the EC50 of Human CD40 (ECD, hFc Tag) is 60-600 ng/mL. |
产品描述 | CD40 Ligand Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 17.7 kDa and the accession number is P29965. |
种属 | Human |
表达系统 | E. coli |
标签 | C-His |
蛋白编号 | P29965 |
别名 | IMD3, hCD40L, CD40L, TRAP, TNFSF5, IGM, HIGM1, gp39, CD154, T-BAM, CD40 Ligand |
蛋白构建 | The soluble form of Human CD40L (NP_000065.1) (Glu 108-Leu 261) was expressed with an initial Met at the N-terminus and a polyhistide tag at the C-terminus. |
蛋白纯度 | ≥ 95 % as determined by SDS-PAGE. ≥ 85 % as determined by SEC-HPLC. |
分子量 | 17.7 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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CD40 Ligand Protein, Human, Recombinant (His) IMD 3 TNFSF-5 IMD3 hCD40L CD40L TRAP IMD-3 TNFSF5 IGM HIGM 1 HIGM1 HIGM-1 TNFSF 5 CD 154 gp39 CD154 T-BAM CD-154 CD40 Ligand recombinant recombinant-proteins proteins protein