Carbonic Anhydrase 2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is P00918.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,520 | 现货 | ||
200 μg | ¥ 4,470 | 5日内发货 |
生物活性 | Measured by its esterase activity. The specific activity is >150 pmoles/min/μg. |
产品描述 | Carbonic Anhydrase 2 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is P00918. |
种属 | Human |
表达系统 | E. coli |
标签 | C-His |
蛋白编号 | P00918 |
别名 | HEL-S-282, CA-II, HEL-76, CAII, carbonic anhydrase II, CAC, Car2 |
蛋白构建 | The Human CA2 (NP_000058.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus. |
蛋白纯度 |
≥ 96 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
|
分子量 | 30 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris 0.5M NaCl, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1. |
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Carbonic Anhydrase 2 Protein, Human, Recombinant (His) HEL-S 282 HEL-S-282 CA-II HEL-76 HEL 76 CAII carbonic anhydrase II HEL-S282 HEL76 CAC Car2 recombinant recombinant-proteins proteins protein