Bikunin/AMBP Protein, Human, Recombinant (aa 206-348, His) is expressed in yeast with His tag. The predicted molecular weight is 17.4 kDa and the accession number is CAA38587.1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,750 | 5日内发货 |
生物活性 | Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK (Dnp)-NH2 . The IC50 value is < 5 nM. |
产品描述 | Bikunin/AMBP Protein, Human, Recombinant (aa 206-348, His) is expressed in yeast with His tag. The predicted molecular weight is 17.4 kDa and the accession number is CAA38587.1. |
种属 | Human |
表达系统 | P. pastoris (Yeast) |
标签 | N-His |
蛋白编号 | CAA38587.1 |
别名 | EDC1, ITILC, UTI, α-1-microglobulin/bikunin precursor, HCP, ITIL, A1M, ITI, alpha-1-microglobulin/bikunin precursor, IATIL, HI30 |
蛋白构建 | Human AMBP (CAA38587.1) (Ala206-Leu348) |
蛋白纯度 | > 85 % as determined by SDS-PAGE. |
分子量 | 17.4 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The AMBP [A1M (alpha1-microglobulin)/bikunin precursor] gene encodes two plasma glycoproteins: A1M, an immunosuppressive lipocalin, and bikunin, a member of plasma serine proteinase inhibitor family with prototypical Kunitz-type domain. Although previously believed to be constitutively expressed exclusively in liver, the present study demonstrates the induction of this gene by oxalate in porcine proximal tubular LLC-PK1 cells and rat kidney. In liver, the precursor protein is cleaved in the Golgi network by a furin-like enzyme to release constituent proteins, which undergo glycosylation before their export from the cell. In the renal tubular cells, A1M and bikunin co-precipitate, indicating lack of cleavage of the precursor protein. As the expression of the AMBP gene is regulated by A1M-specific cis elements and transcription factors, A1M protein was studied as a representative of AMBP gene expression in renal cells. The alpha(1)-microglobulin/bikunin precursor (AMBP) gene, and its two protein products were studied in mouse embryos of 8.5-15.5 days of embryonic development by in situ hybridization and immunohistochemistry. AMBP mRNA is strongly transcribed in liver parenchyma, pancreas, and intestine epithelium. Sites of weaker expression are the vessels of the umbilical cord, the developing vertebral bodies, and kidney. The alpha(1)-microglobulin and bikunin proteins are accordingly present in developing hepatocytes, pancreas, kidney, and gut. However, additional sites of protein distribution were found that do not correlate to mRNA localization: alpha(1)-microglobulin was found in myocytes and bikunin in cardiac muscle, nervous system microvasculature, and connective tissue |
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Bikunin/AMBP Protein, Human, Recombinant (aa 206-348, His) EDC1 ITILC EDC 1 UTI α-1-microglobulin/bikunin precursor HCP ITIL A1M ITI alpha-1-microglobulin/bikunin precursor HI 30 a-1-microglobulin/bikunin precursor HI-30 EDC-1 IATIL HI30 recombinant recombinant-proteins proteins protein