Powder: -20°C for 3 years | In solvent: -80°C for 1 year
X-NeuNAc (5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt) 是细菌表达系统中神经氨酸酶活性的一种新的显色测定底物。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 293 | 现货 | ||
5 mg | ¥ 417 | 现货 | ||
10 mg | ¥ 597 | 现货 | ||
25 mg | ¥ 1,238 | 现货 | ||
50 mg | ¥ 1,998 | 现货 | ||
100 mg | ¥ 2,998 | 现货 | ||
200 mg | ¥ 3,980 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 566 | 现货 |
产品描述 | X-NeuNAc (5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt) is a new chromogenic assay substrate of neuraminidase activity in bacterial expression systems. |
体外活性 | X-Neu5Ac was hydrolyzed by neuraminidase to release a halogenated product undergoing rapid aerobic oxidation to form the dark blue pigment. Preliminary kinetic studies indicated that X-Neu5Ac was a good and stable substrate for neuraminidase. In addition, X-Neu5Ac would also be hydrolyzed mutant enzymes |
体内活性 | To visualize extracellular sialidase activity on the membrane surface in the rat brain, acute brain slices were incubated with X-Neu5Ac at pH 7.3. After 1h, myelin-abundant regions showed intense fluorescence in the rat brain. Although the hippocampus showed weak fluorescence in the brain, mossy fiber terminals in the hippocampus showed relatively intense fluorescence. In addition, the fluorescence intensities caused by X-Neu5Ac was correlated with the sialidase activity. Therefore, staining with X-Neu5Ac was specific for sialidase and useful for quantitative analysis of sialidase activities [1]. |
别名 | X-Neu5Ac, 5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt |
分子量 | 559.72 |
分子式 | C19H21BrClN2NaO9 |
CAS No. | 160369-85-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 60 mg/ml (107.2 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.7866 mL | 8.933 mL | 17.8661 mL | 44.6652 mL |
5 mM | 0.3573 mL | 1.7866 mL | 3.5732 mL | 8.933 mL | |
10 mM | 0.1787 mL | 0.8933 mL | 1.7866 mL | 4.4665 mL | |
20 mM | 0.0893 mL | 0.4467 mL | 0.8933 mL | 2.2333 mL | |
50 mM | 0.0357 mL | 0.1787 mL | 0.3573 mL | 0.8933 mL | |
100 mM | 0.0179 mL | 0.0893 mL | 0.1787 mL | 0.4467 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
X-NeuNAc 160369-85-7 Others X-Neu5Ac XNeuNAc X NeuNAc 5-Bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid sodium salt Inhibitor inhibitor inhibit