store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NSC668394 是一种有效的小分子 ezrin (Thr567) 磷酸化抑制剂。NSC668394 具有潜在的抗肿瘤活性,抑制 ezrin T567 磷酸化,增加 Ca2+依赖性ezrin裂解。NSC668394 可用于研究肿瘤转移。
产品描述 | NSC668394 is a potent small molecule ezrin (Thr567) phosphorylation inhibitor.NSC668394 has potential antitumor activity by inhibiting ezrin T567 phosphorylation and increasing Ca2+-dependent ezrin cleavage.NSC668394 can be used to study tumor metastasis. |
靶点活性 | Ezrin T567:8.1 μM |
体外活性 |
NSC668394, when pretreated at 10 μM for 15 minutes, inhibits ezrin T567 phosphorylation (IC50=8.1 μM) and in vitro actin binding[2]. Additionally, at concentrations ranging from 1 to 10 μM for 2 to 6 hours, NSC668394 inhibits ezrin-mediated invasion of K7M2 osteosarcoma (OS) cells into HUVEC monolayers[2]. Furthermore, at a concentration of 20 μM, NSC668394 significantly reduces the growth of JM1 and JM2 rat hepatocellular carcinoma cell lines[1]. Notably, NSC668394 at 10 μM diminishes cell motility phenotypes in zebrafish[2]. |
体内活性 | Administered at a dose of 0.226 mg/kg/day through weekly intraperitoneal injections for 5 days, NSC668394 inhibits ezrin protein-dependent in vivo metastatic growth in the lungs of mice[2]. |
别名 | NSC-668394, NSC 668394 |
分子量 | 452.1 |
分子式 | C17H12Br2N2O3 |
CAS No. | 382605-72-3 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 30 mg/mL(66.36 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.2119 mL | 11.0595 mL | 22.119 mL | 55.2975 mL |
5 mM | 0.4424 mL | 2.2119 mL | 4.4238 mL | 11.0595 mL | |
10 mM | 0.2212 mL | 1.106 mL | 2.2119 mL | 5.5298 mL | |
20 mM | 0.1106 mL | 0.553 mL | 1.106 mL | 2.7649 mL | |
50 mM | 0.0442 mL | 0.2212 mL | 0.4424 mL | 1.106 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NSC668394 382605-72-3 Chromatin/Epigenetic Cytoskeletal Signaling PKC NSC-668394 NSC 668394 Inhibitor inhibitor inhibit