SPG21 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 62.8kDa and the accession number is Q9CQC8-1.
生物活性 | Testing in progress |
产品描述 | SPG21 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 62.8kDa and the accession number is Q9CQC8-1. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | Q9CQC8-1 |
别名 | ACP33, C78576, BM-019, GL010, D9Wsu18e, MAST, spastic paraplegia 21 (autosomal recessive, Mast syndrome) |
蛋白构建 | The Mouse SPG21 isoform 1 (Q9CQC8-1) (Met 1-Pro 308) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | > 90 % as determined by SDS-PAGE |
分子量 | 62.8kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 3 mM DTT, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Spastic paraplegia 21 (SPG21), also known as acid Cluster Protein 33 (ACP33) and Mast syndrome protein, is a member of the AB hydrolase superfamily. Human SPG21 is a 38 amino acid residue protein widely expressed in all tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. SPG21 binds to the hydrophobic C-terminal amino acids of CD4 which are involved in repression of T cell activation via the noncatalytic alpha/beta hydrolase fold domain. SPG21 thus is proposed to play a role as a negative regulatory factor in CD4-dependent T-cell activation of CD4. Defects in SPG21 are the cause of spastic paraplegia autosomal recessive type 21, also known as Mast syndrome, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. SPG21 is also associated with dementia and other central nervous system abnormalities. |
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SPG21 Protein, Mouse, Recombinant (His & GST) BM 019 ACP33 GL 010 ACP 33 C78576 BM-019 C 78576 autosomal recessive, Mast syndrome GL010 spastic paraplegia 21 C-78576 ACP-33 D9Wsu18e MAST spastic paraplegia 21 (autosomal recessive, Mast syndrome) BM019 GL-010 recombinant recombinant-proteins proteins protein