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SARS-CoV Spike/S1 Protein (His)

产品编号 TMPY-03988

别名: ncov RBD Protein, SARS, nove, ncov s1 Protein, SARS, coronavirus spike Protein, SARS, coronavirus s1 Protein, SARS, novel coronavirus RBD Protein, SARS, ncov s2 Protein, SARS, cov spike Protein, SARS, ncov spike Protein, SARS, coronavirus s2 Protein, SARS

SARS-CoV Spike/S1 Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 74.4 kDa and the accession number is Q5DIC5.

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SARS-CoV Spike/S1 Protein (His)

规格	价格/CNY		货期	数量
100 μg	￥ 5,750		5日内发货

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参考文献

生物活性	Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2μg/mL (100μL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1), the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, His Tag)(40150-V08B1) is 400-850 ng/mL.
产品描述	SARS-CoV Spike/S1 Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 74.4 kDa and the accession number is Q5DIC5.

种属	SARS
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	Q5DIC5
别名	ncov RBD Protein, SARS, nove, ncov s1 Protein, SARS, coronavirus spike Protein, SARS, coronavirus s1 Protein, SARS, novel coronavirus RBD Protein, SARS, ncov s2 Protein, SARS, cov spike Protein, SARS, ncov spike Protein, SARS, coronavirus s2 Protein, SARS
蛋白构建	S1 subunit of SARS-CoV (isolate:WH20) spike (AAX16192.1) (Met1-Arg667)
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	74.4 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

参考文献

1. Xiao X,et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30. 2. Shen S,et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35. 3. Du L,et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.

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Keywords

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