规格

价格

库存

数量

10 μg

¥ 939

6-8日内发货

20 μg

¥ 1,590

5日内发货

50 μg

¥ 3,670

5日内发货

生物活性

产品描述	NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not the cell cycle-regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for the NEK3 gene. NEK3 may play a role in mitotic regulation.
生物活性	The specific activity was determined to be 8 nmol/min/mg using MBP as substrate.
研究背景	NEK3 (NIMA (never in mitosis gene a)-related expressed kinase 3), contains 1 protein kinase domain and is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. Members of the NEK family of protein kinases share high amino acid homology with NIMA (never in mitosis gene a). NEK3 differs from other NimA family members in that it is not the cell cycle-regulated and is found primarily in the cytoplasm. It is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase. NEK3 mRNA can be detected in all the proliferating cell lines with the amount not changing during the cell cycle. Prolactin stimulates interaction between NEK3 and paxillin leading to increased paxillin phosphorylation, Analysis of breast tissue microarrays show a significant up-regulation of NEK3 expression in malignant versus normal specimens. Multiple transcript variants encoding different isoforms have been found for the NEK3 gene. NEK3 may play a role in mitotic regulation.
种属	Mouse
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	ABK42288.1
蛋白构建	A DNA sequence encoding the mouse NEK3 (ABK42288.1) (Met 1-Ala 509) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. Predicted N terminal: Met
蛋白纯度	> 90 % as determined by SDS-PAGE
蛋白性状	Solution
缓冲液	Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10% glycerol.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
别名	NIMA-related kinase 3
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	84.8 kDa (predicted); 72 kDa (reducing conditions)

储存&溶解度

运输方式

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.

存储

It is recommended to store the product under sterile conditions at -20°C to -80°C. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

NEK3 Protein, Mouse, Recombinant (His & GST)

NEK3 Protein, Mouse, Recombinant (His & GST)

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