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MEK1 Protein, Mouse, Recombinant (His & GST)

产品编号 TMPY-04569

别名: MEKK1, mitogen-activated protein kinase kinase 1, Prkmk1, MAPKK1, Mek1

MEK1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 71.3 kDa and the accession number is P31938.

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MEK1 Protein, Mouse, Recombinant (His & GST)

规格	价格/CNY		货期	数量
50 μg	￥ 2,530		5日内发货

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参考文献

生物活性	No Kinase Activity
产品描述	MEK1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 71.3 kDa and the accession number is P31938.

种属	Mouse
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	P31938
别名	MEKK1, mitogen-activated protein kinase kinase 1, Prkmk1, MAPKK1, Mek1
蛋白构建	The Mouse MAP2K1 (P31938) (Met 1-Ile 393) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	71.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% gly, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	MEK1, also known as MAP2K1 and MKK1, is a member of the dual-specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. MEK1 is widely expressed, with extremely low levels in the brain. It lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcription regulation, and development. Binding extracellular ligands such as growth factors, cytokines, and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1 and MEK2. MEK1 has been shown to export PPARG from the nucleus. The MAPK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis. MKK1 catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Defects in MEK1 can cause Cardiofaciocutaneous Syndrome.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Rampoldi L,et al.(1998) Chromosomal localization of four MAPK signaling cascade genes: MEK1, MEK3, MEK4 and MEKK5. Cytogenet Cell Genet. 78(3-4):301-3. 2. Zheng CF,et al.(1993) Cloning and characterization of two distinct human extracellular signal-regulated kinase activator kinases, MEK1 and MEK2. J Biol Chem. 268(15):11435-9. 3. Nantel,et al.(1998) Interaction of the Grb10 adapter protein with the Raf1 and MEK1 kinases. J Biol Chem. 273(17):10475-84. 4. Hirata H,et al.(2012) I MicroRNA-1826 targets VEGFC, beta-catenin (CTNNB1) and MEK1 (MAP2K1) in human bladder cancer. Carcinogenesis. 33(1):41-8.

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Keywords

MEK1 Protein, Mouse, Recombinant (His & GST) MEKK1 MAPKK 1 mitogen-activated protein kinase kinase 1 Prkmk1 MEKK 1 MAPKK1 MEKK-1 Mek1 MAPKK-1 recombinant recombinant-proteins proteins protein

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