Integrin alpha 6 beta 4 Protein, Human, Recombinant (Flag & His) is expressed in HEK293 mammalian cells with Flag and His tag. The predicted molecular weight is 196 kDa and the accession number is CAA37656.1.

Human

HEK293 Cells

C-Flag, C-His

A DNA sequence encoding the extracellular domain (Met 1-Ser 1011) of human ITGA6 isoform alpha-6X1B (P23229-3) was fused with a flag tag at the C-terminus, constructed the plasmid 1 ; A DNA sequence encoding the extracellular domain (Met 1-Ser 710) of human ITGB4 (CAA37656.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified Predicted N terminal: Phe 24 & Asn 28

Lyophilized powder

Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.

Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.

Integrin α6 β 4, Integrin alpha 6 β4