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EphB1 Protein, Human, Recombinant (aa 565-984, His & GST)

EphB1 Protein, Human, Recombinant (aa 565-984, His & GST)

产品编号 TMPY-04416
别名: EPH receptor B1, EPHT2, ELK, Hek6, NET

EphB1 Protein, Human, Recombinant (aa 565-984, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.3 kDa and the accession number is AAI11745.1.

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EphB1 Protein, Human, Recombinant (aa 565-984, His & GST)
规格 价格/CNY 货期 数量
50 μg ¥ 3,170 5日内发货
药物设计专题培训
千万补贴 助力科研
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产品目录号及名称: EphB1 Protein, Human, Recombinant (aa 565-984, His & GST) (TMPY-04416)
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参考文献
生物活性 The specific activity was determined to be 140 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.
产品描述 EphB1 Protein, Human, Recombinant (aa 565-984, His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.3 kDa and the accession number is AAI11745.1.
种属 Human
表达系统 Baculovirus Insect Cells
标签 His, GST
蛋白编号 AAI11745.1
别名 EPH receptor B1, EPHT2, ELK, Hek6, NET
蛋白构建 The Human EPHB1 (AAI11745.1) (Arg565-Ala984) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度 > 95 % as determined by SDS-PAGE
分子量 75.3 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Supplied as sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, pH 8.0, 10% gly.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice.

研究背景 Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

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Keywords

EphB1 Protein, Human, Recombinant (aa 565-984, His & GST) EPH receptor B1 EPHT 2 EPHT2 ELK Hek6 NET EPHT-2 recombinant recombinant-proteins proteins protein

 

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