DDR1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.8kDa and the accession number is Q03146-2.
生物活性 | The specific activity was determined to be 2 nmol/min/mg using synthetic modified AXLtide peptide (modified-CKKSRGDYMTMQIG) as substrate. |
产品描述 | DDR1 Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 75.8kDa and the accession number is Q03146-2. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | Q03146-2 |
别名 | 6030432F18, Nep, Cak, AI323681, CD167a, discoidin domain receptor tyrosine kinase 1, PTK3A |
蛋白构建 | The Mouse DDR1 (Q03146-2) (Leu444-Val874) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | ≥ 85 % as determined by SDS-PAGE |
分子量 | 75.8kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 2 mM DTT. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
研究背景 | Discoidin domain receptor family, member 1 (DDR1), also known as or CD167a (cluster of differentiation 167a), and Mammary carcinoma kinase 10 (MCK10), belongs to a subfamily of tyrosine kinase receptors with an extracellular domain homologous to Dictyostellium discoideum protein discoidin 1. Receptor tyrosine kinases play a key role in the communication of cells with their microenvironment. These kinases are involved in the regulation of cell growth, differentiation and metabolism. Expression of DDR1/MCK10/CD167 is restricted to epithelial cells, particularly in the kidney, lung, gastrointestinal tract, and brain. In addition, it has been shown to be significantly overexpressed in several human tumors. DDR1/MCK10/CD167 plays an important role in regulating attachment to collagen, chemotaxis, proliferation, and MMP production in smooth muscle cells. DDR1 functions in a feedforward loop to increase p53 levels and at least some of its effectors. Inhibition of DDR1 function resulted in strikingly increased apoptosis of wild-type p53-containing cells in response to genotoxic stress through a caspase-dependent pathway. |
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DDR1 Protein, Mouse, Recombinant (His & GST) AI-323681 6030432F18 Nep Cak AI323681 AI 323681 CD167a discoidin domain receptor tyrosine kinase 1 PTK3A recombinant recombinant-proteins proteins protein