Coagulation Factor II Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.3 kDa and the accession number is P19221.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
20 μg | ¥ 2,270 | 5日内发货 |
生物活性 | Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is > 2000 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form) |
产品描述 | Coagulation Factor II Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 69.3 kDa and the accession number is P19221. |
种属 | Mouse |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | P19221 |
别名 | Cf-2, coagulation factor II (thrombin), Cf2, FII |
蛋白构建 | The full length of Mouse F2 (NP_034298.1) (Met 1-Gly 618) was expressed, with a C-terminal polyhistidine tag. |
蛋白纯度 | > 96 % as determined by SDS-PAGE |
分子量 | 69.3 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Coagulation Factor II Protein (FII, F2 Protein or Prothrombin) is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. Coagulation Factor II Protein (FII, F2 Protein) also plays a role in maintaining vascular integrity during development and postnatal life. Prothrombin / Coagulation Factor II is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin / Coagulation Factor II and factor Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin. Prothrombin / Coagulation Factor II is expressed by the liver and secreted in plasma. Defects in prothrombin / Coagulation Factor II are the cause of factor II deficiency (FA2D). It is very rare blood coagulation disorder characterized by mucocutaneous bleeding symptoms. The severity of the bleeding manifestations correlates with blood factor II levels. Defects in Coagulation Factor II are also a cause of susceptibility to thrombosis. It is a multifactorial disorder of hemostasis characterized by abnormal platelet aggregation in response to various agents and recurrent thrombi formation. |
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Coagulation Factor II Protein, Mouse, Recombinant (His) Cf-2 thrombin coagulation factor II (thrombin) coagulation factor II Cf2 FII recombinant recombinant-proteins proteins protein