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CD3D Protein, Human, Recombinant (hFc & FLAG), Biotinylated

产品编号 TMPY-05243

别名: CD3 delta, CD3-δ, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex), CD3 δ, CD3-DELTA, IMD19, T3D

CD3D Protein, Human, Recombinant (hFc & FLAG), Biotinylated is expressed in HEK293 mammalian cells with hFc and Flag tag. The predicted molecular weight is 37.8 kDa and the accession number is B0YIY4.

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CD3D Protein, Human, Recombinant (hFc & FLAG), Biotinylated

规格	价格/CNY		货期	数量
20 μg	￥ 2,440		5日内发货
100 μg	￥ 5,810		5日内发货

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生物活性	Testing in progress
产品描述	CD3D Protein, Human, Recombinant (hFc & FLAG), Biotinylated is expressed in HEK293 mammalian cells with hFc and Flag tag. The predicted molecular weight is 37.8 kDa and the accession number is B0YIY4.

种属	Human
表达系统	HEK293 Cells
标签	hFc, Flag
蛋白编号	B0YIY4
别名	CD3 delta, CD3-δ, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex), CD3 δ, CD3-DELTA, IMD19, T3D
蛋白构建	The Human CD3D (NP_000723.1) (Met1-Ala105) was fused with the C-terminal flag-tagged Fc region of Human IgG1 at the C-terminus. The purified protein was biotinylated in vitro.
蛋白纯度	> 80 % as determined by SDS-PAGE
分子量	37.8 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	T-cell surface glycoprotein CD3 delta chain, also known as CD3D, is a single-pass type I membrane protein. CD3D, together with CD3-gamma, CD3-epsilon and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The majority of T cell receptor (TCR) complexes in mice and humans consist of a heterodimer of polymorphic TCR alpha and beta chains along with invariant CD3 gamma, delta, epsilon, and zeta chains. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). CD3D contains 1 ITAM domain and has been shown to interact with CD8A. In the mouse, knockout of CD3delta allows some degree of T lymphocyte differentiation since mature CD4 and CD8 as well as TCRgammadelta T lymphocytes are observed in the periphery. In contrast, deleterious mutation of the CD3 delta encoding gene in the human leads to a severe combined immunodeficiency characterised by the complete absence of mature T cell subpopulations including TCRalpha/beta and TCR gamma/delta. Defects in CD3D cause severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-/B+/NK+ SCID) which is a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. In humans the absence of CD3 delta results in a complete arrest in thymocyte development at the stage of double negative to double positive transition and the development of gamma delta T-cell receptor-positive T cells is also impaired.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy

参考文献

1. Roifman CM. (2004) CD3 delta immunodeficiency. Curr Opin Allergy Clin Immunol. 4(6): 479-84. 2. Pan Q,et al.(2006) Different role for mouse and human CD3delta/epsilon heterodimer in preT cell receptor (preTCR) function: human CD3delta/epsilon heterodimer restores the defective preTCR function in CD3gamma- and CD3gammadelta-deficient mice. Mol Immunol. 43(11): 1741-50. 3. Le Deist F,et al.(2007) Expression anomalies of the CD3-TCR complex expression and immunodeficiencies. Med Sci (Paris). 23(2): 161-6.

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Keywords

CD3D Protein, Human, Recombinant (hFc & FLAG), Biotinylated CD3-TCR complex CD3 delta CD3-δ IMD-19 CD3d molecule, δ (CD3-TCR complex) CD3d molecule, delta (CD3-TCR complex) CD3 δ CD3d molecule, delta CD3-DELTA IMD19 IMD 19 T3D recombinant recombinant-proteins proteins protein

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