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CD16a Protein, Human, Recombinant (176 Phe, His & Avi)

CD16a Protein, Human, Recombinant (176 Phe, His & Avi)

产品编号 TMPY-01965
别名: IMD20, IGFR3, FCGR3, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc γ RIIIa, CD16, FCR-10, FCG3, CD16A, FCRIIIA, FCGRIII, Fc gamma RIIIa, FCRIII

CD16a Protein, Human, Recombinant (176 Phe, His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.1 kDa and the accession number is P08637-1.

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CD16a Protein, Human, Recombinant (176 Phe, His & Avi)
规格 价格/CNY 货期 数量
100 μg ¥ 3,820 5日内发货
1 mg ¥ 24,900 5日内发货
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产品目录号及名称: CD16a Protein, Human, Recombinant (176 Phe, His & Avi) (TMPY-01965)
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生物活性
技术参数
产品性质
参考文献
生物活性 Measured by its binding ability in a functional ELISA. Immobilized Human CD16a His & AVI at 2 μg/ml (100 μl/well) can bind Human IgG1 , the EC50 of Human IgG1 is 0.4-1.2 μg/mL.
产品描述 CD16a Protein, Human, Recombinant (176 Phe, His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.1 kDa and the accession number is P08637-1.
种属 Human
表达系统 HEK293 Cells
标签 His, Avi
蛋白编号 P08637-1
别名 IMD20, IGFR3, FCGR3, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), Fc γ RIIIa, CD16, FCR-10, FCG3, CD16A, FCRIIIA, FCGRIII, Fc gamma RIIIa, FCRIII
蛋白构建 The extracellular domain (Met 1-Gln 208) of Human CD16a (P08637-1) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
蛋白纯度 > 95 % as determined by SDS-PAGE
分子量 25.1 kDa (predicted)
内毒素 < 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.2. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法 A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice.

研究背景 The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.

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Keywords

CD16a Protein, Human, Recombinant (176 Phe, His & Avi) IMD20 IMD 20 FCGR-3 IGFR 3 IGFR3 Fc fragment of IgG, low affinity IIIa, receptor FCGR3 CD16a CD 16 Fc gamma RIIIa FCR 10 Fc fragment of IgG, low affinity IIIa, receptor (CD16a) FCG 3 Fc γ RIIIa CD16 CD-16 IMD-20 FCG-3 FCR-10 FCG3 CD16A FCRIIIA FCGRIII Fc gamma RIIIa FCGR 3 FCR10 FCRIII IGFR-3 recombinant recombinant-proteins proteins protein

 

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