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B4GALT1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-06809

别名: Ggtb, β4Gal-T1, B-1,4-GalT, GalT, β-1,4-GalT1, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1, B-1,4-GalT1, b4Gal-T1, beta-1,4-GalT, β-1,4-GalT, UDP-Gal:βGlcNAc β 1,4- galactosyltransferase, polypeptide 1, beta-1,4-GalT1, Ggtb2, beta4Gal-T1

B4GALT1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 41.12 kDa and the accession number is P15535.

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B4GALT1 Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
100 μg	￥ 7,680		5日内发货
1 mg	￥ 50,000		5日内发货

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生物活性	Testing in progress
产品描述	B4GALT1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 41.12 kDa and the accession number is P15535.

种属	Mouse
表达系统	HEK293 Cells
标签	N-His
蛋白编号	P15535
别名	Ggtb, β4Gal-T1, B-1,4-GalT, GalT, β-1,4-GalT1, UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1, B-1,4-GalT1, b4Gal-T1, beta-1,4-GalT, β-1,4-GalT, UDP-Gal:βGlcNAc β 1,4- galactosyltransferase, polypeptide 1, beta-1,4-GalT1, Ggtb2, beta4Gal-T1
蛋白构建	The Mouse B4GALT1 (P15535) (Gly44-Arg399) was expressed, with a polyhistidine tag at the N-terminus.
蛋白纯度	≥ 95 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
分子量	41.12 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.

参考文献

1. Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95. 2. Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34. 3. Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.

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Keywords

B4GALT1 Protein, Mouse, Recombinant (His) Ggtb β4Gal-T1 B-1,4-GalT GalT β-1,4-GalT1 UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1 B-1,4-GalT1 b4Gal-T1 beta-1,4-GalT β-1,4-GalT UDP-Gal:βGlcNAc β 1,4- galactosyltransferase, polypeptide 1 beta-1,4-GalT1 Ggtb2 beta4Gal-T1 recombinant recombinant-proteins proteins protein

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