Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 79.33 kDa and the accession number is P10696.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,100 | 5日内发货 | ||
500 μg | ¥ 12,400 | 5日内发货 | ||
1 mg | ¥ 20,700 | 5日内发货 |
生物活性 | Immobilized Human Alkaline Phosphatase (Germ Type) , hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Alkaline Phosphatase Antibody, hFc Tag with the EC50 of 15.4ng/ml determined by ELISA. |
产品描述 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 79.33 kDa and the accession number is P10696. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc |
蛋白编号 | P10696 |
别名 | GCAP, ALPPL, Alkaline phosphatase, placental-like, Alkaline Phosphatase, ALP-1, PLAP-like, ALPG, ALPPL2 |
蛋白构建 | Ile20-Asp503 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 79.33 kDa (predicted). Due to glycosylation, the protein migrates to 80-100 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 25 mM Tris, 100 mM NaCl, 1 mM MgCl2 (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Alkaline phosphatase can be considered "our favorite enzyme" for reasons apparent to those who diagnose and treat metabolic bone diseases or who study skeletal biology. Few might know, however, that alkaline phosphatase likely represents the most frequently assayed enzyme in all of medicine. Elevated activity in the circulation is universally recognized as a marker for skeletal or hepatobiliary disease. |
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Alkaline Phosphatase (Germ type) /ALPG Protein, Human, Recombinant (hFc) ALP 1 GCAP ALPPL Alkaline phosphatase, placental-like Alkaline Phosphatase ALP-1 ALPPL-2 ALP1 ALPPL 2 PLAP-like ALPG ALPPL2 recombinant recombinant-proteins proteins protein