4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (Avi & Fc), Biotinylated

4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (Avi & Fc), Biotinylated is expressed in HEK293 mammalian cells with AVI and Fc tag. The predicted molecular weight is 47.98 kDa and the accession number is A0A0U5J8I0.

规格	价格/CNY		货期	数量
20 μg	￥ 2,940	5日内发货
100 μg	￥ 6,800	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,940

5日内发货

100 μg

￥ 6,800

5日内发货

生物活性	1. Immobilized 4-1BB-Fch at 10 μg/mL (100 μL/well) can bind S3-Avi-Fc-TNFSF9 , the EC50 of S3-Avi-Fc-TNFSF9 is 7.0-20 ng/mL. 2. Measured by it's ability to induce IL-8 secretion by HT1080 transfected with human CD137. The ED50 for this effect is typically 0.25-1.5 μg/mL.
产品描述	4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (Avi & Fc), Biotinylated is expressed in HEK293 mammalian cells with AVI and Fc tag. The predicted molecular weight is 47.98 kDa and the accession number is A0A0U5J8I0.

种属	Human
表达系统	HEK293 Cells
标签	N-Avi-hFc
蛋白编号	A0A0U5J8I0
别名	4-1BBL, 4-1BB-L, CD137L, tumor necrosis factor (ligand) superfamily, member 9
蛋白构建	The Human TNFSF9 (NP_003802.1) (Arg71-Glu254) was expressed with a N-terminal AVI tag followed by a Fc region of Human IgG1 tag. The purified protein was biotinylated in vitro.
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	47.98 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy