Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Thioflavin T (Basic Yellow 1) 是阳离子 Benzothiazole 染料,在组织切片中能够淀粉样蛋白结合,增强荧光强度。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
500 mg | ¥ 150 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 232 | 现货 |
产品描述 | Thioflavin T (Basic Yellow 1) is a widely used dye for visualizing and is a cationic Benzothiazole dye that shows enhanced fluorescence upon binding to amyloid in tissue sections. |
体外活性 | Thioflavin T (ThT) is a benzothiazole dye. The Thioflavin T (ThT) exhibits enhanced fluorescence upon binding to amyloid fibrils and is commonly used to diagnose amyloid fibrils, both ex vivo and in vitro. Changes in the fluorescence excitation and emission of Thioflavin T are also dependent on the micelle formation. The Thioflavin T micelles of 3 nm diameter are directly visualized using atomic force microscopy, and bound Thioflavin T micelles are observed along the fibril length for representative fibrils. The micelles of Thioflavin T bind amyloid fibrils leading to enhancement of fluorescence emission[1]. In aqueous solutions, Thioflavin T is found to exist as micelles at concentrations commonly used to monitor fibrils by fluorescence assay (~10-20 μM). Specific conductivity changes are measured at varying concentration of Thioflavin T and the critical micellar concentration is calculated to be 4.0±0.5 μM. Increasing concentration of Thioflavin T above the critical micellar concentration shows increased numbers of micelles bound along the length of the amyloid fibrils. Thioflavin T micelles are disrupted at low pH as observed by atomic force microscopy and fluorescence enhancement upon binding of Thioflavin T to amyloid fibrils also reduced by several-fold upon decreasing the pH to below 3. |
别名 | Basic Yellow 1, 硫黄素 T, 硫黄素T |
分子量 | 318.86 |
分子式 | C17H19ClN2S |
CAS No. | 2390-54-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 12.5 mg/mL (39.20 mM), Sonication is recommended.
DMSO: 21.5 mg/mL(67.43 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 3.1362 mL | 15.6809 mL | 31.3617 mL | 78.4043 mL |
5 mM | 0.6272 mL | 3.1362 mL | 6.2723 mL | 15.6809 mL | |
10 mM | 0.3136 mL | 1.5681 mL | 3.1362 mL | 7.8404 mL | |
20 mM | 0.1568 mL | 0.784 mL | 1.5681 mL | 3.9202 mL | |
DMSO | 50 mM | 0.0627 mL | 0.3136 mL | 0.6272 mL | 1.5681 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Thioflavin T 2390-54-7 Others Inhibitor Basic Yellow 1 Basic Yellow-1 inhibit Basic Yellow1 硫黄素 T 硫黄素T inhibitor