Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Soyasaponin Ac can decrease the drug resistance via EMT pathway and weaken the migration ability of ovarian cancer cells.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
5 mg | ¥ 2,380 | 待询 | ||
1 mL * 10 mM (in DMSO) | ¥ 5,440 | 待询 |
产品描述 | Soyasaponin Ac can decrease the drug resistance via EMT pathway and weaken the migration ability of ovarian cancer cells. |
体外活性 | Cell viability assay was used to analyze the drug resistance of A2780/PTX cells treated with four different common soyasaponins respectively,in order to screen out the most effective soyasaponin. Then,the most effective soyasaponin was used to detect the expression of epithelial-mesenchymal transition (EMT)-related marker proteins, including N-cadherin and E-cadherin,with Western blot and confocal microscopy. Finally,transwell assay and wound healing assay were applied to observe effect of soyasaponin on regulating cancer cell migration. Compared with other soyasaponins,Soyasaponin Ac most effectively reversed the drug resistance of A2780/PTX cells. The expression of N-cadherin decreased while that of Ecadherin increased in A2780/PTX cells when treated with Soyasaponin Ac for 48 h. The results of transwell and wound healing assay suggested that Soyasaponin Ac also reduced the migration of A2780/PTX cells. |
分子量 | 1421.52 |
分子式 | C67H104O32 |
CAS No. | 133882-74-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Soyasaponin Ac 133882-74-3 Others Inhibitor inhibitor inhibit