Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MAC13243 HCl 是一种将脂蛋白从内膜运输到外膜的周质伴侣LolA的抑制剂,是一种具有特异性的抗菌剂,对革兰氏阴性菌抑制作用显著。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
1 mg | ¥ 959 | 现货 | ||
5 mg | ¥ 2,380 | 现货 | ||
10 mg | ¥ 3,530 | 现货 | ||
25 mg | ¥ 5,730 | 现货 | ||
50 mg | ¥ 7,970 | 现货 | ||
100 mg | ¥ 10,700 | 现货 | ||
500 mg | ¥ 21,500 | 现货 |
产品描述 | MAC13243 HCl, an inhibitor of LolA, a periplasmic chaperone that transports lipoproteins from the inner membrane to the outer membrane, is an antimicrobial agent with specificity and significant inhibitory effects on Gram-negative bacteria. |
体外活性 | We investigated the impact of a prototype drug (MAC13243 HCl) interfering with the Gram-negative outer membrane protein LolA on the faecal microbiota. Faecal suspensions from two healthy human donors were exposed to MAC13243 HCl (16, 32, or 64 mg/L) using an in vitro gut model (CoMiniGut). Samples collected 0, 4, and 8 h after exposure were subjected to viable cell counts, 16S rRNA gene quantification and V3-V4 sequencing using the Illumina MiSeq platform. MAC13243 HCl exhibited concentration-dependent killing of coliforms in both donors after 8 h. Concentrations of ≤32 mg/L reduced the growth of aerobic bacteria without influencing the microbiota composition and diversity. An expansion of Firmicutes at the expense of Bacteroidetes and Actinobacteria was observed in the faecal microbiota from one donor following exposure to 64 mg/L of MAC13243 HCl. At all concentrations tested, MAC13243 HCl did not lead to the proliferation of Escherichia coli nor a reduced abundance of beneficial bacteria, which are typical changes observed in antibiotic-induced dysbiosis. These results support our hypothesis that a drug interfering with a specific target in Gram-negative bacteria has a low impact on the commensal gut microbiota and, therefore, a low risk of inducing dysbiosis.[3] |
分子量 | 442.4 |
分子式 | C20H25Cl2N3O2S |
CAS No. | 1071638-38-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (113.02 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 2.2604 mL | 11.302 mL | 22.604 mL | 56.5099 mL |
5 mM | 0.4521 mL | 2.2604 mL | 4.5208 mL | 11.302 mL | |
10 mM | 0.226 mL | 1.1302 mL | 2.2604 mL | 5.651 mL | |
20 mM | 0.113 mL | 0.5651 mL | 1.1302 mL | 2.8255 mL | |
50 mM | 0.0452 mL | 0.226 mL | 0.4521 mL | 1.1302 mL | |
100 mM | 0.0226 mL | 0.113 mL | 0.226 mL | 0.5651 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
MAC13243 HCl 1071638-38-4 Microbiology/Virology Antibacterial MAC-13243 MAC13243 Inhibitor inhibitor inhibit