store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Forodesine is a potent human lymphocyte proliferation inhibitor. Forodesine is an orally active inhibitor of purine nucleoside phosphorylase (IC50s: 0.48 to 1.57 nM for human, mouse, rat, monkey, and dog PNP). It could induce apoptosis in leukemic cells by increasing the dGTP levels.
产品描述 | Forodesine is a potent human lymphocyte proliferation inhibitor. Forodesine is an orally active inhibitor of purine nucleoside phosphorylase (IC50s: 0.48 to 1.57 nM for human, mouse, rat, monkey, and dog PNP). It could induce apoptosis in leukemic cells by increasing the dGTP levels. |
靶点活性 | PNP (human):1.19 nM, PNP (monkey):0.66 nM, PNP (mouse):0.48 nM, PNP (rat):1.24 nM, PNP (dog):1.57 nM |
体外活性 | Forodesine (10-30?μM; 24 and 48 hours; RPMI-8226, MOLT-4, and 5T33MM cells) has no effect on the MM cells at 24 hours, while it could decrease the percentage of living cells in the MOLT-4 cells with 40%. Forodesine (10-30?μM; 24 and 48 hours; RPMI-8226, MOLT-4, and 5T33MM cells) treatment is partially inhibition of proliferation [1]. Forodesine inhibits human lymphocyte proliferation activated by various agents such as interleukin-2 (IL-2), mixed lymphocyte reaction (MLR) and phytohemagglutinin (PHA) (IC50s < 0.1-0.38 μM), in the presence of 2'-deoxyguanosine (dGuo, 3-10 μM) [2]. |
体内活性 | Forodesine has excellent oral bioavailability (63%) in mice. Forodesine(single dose of 10 mg/kg; in mice ) treatment, elevates dGuo to approximately 5 μM. Forodesine is effective in prolonging the life span 2-fold or more, in the human peripheral blood lymphocyte severe combined immunodeficiency (hu-PBL-SCID) mouse model [2]. |
别名 | 呋咯地辛, BCX-1777, Immucillin-H |
分子量 | 266.25 |
分子式 | C11H14N4O4 |
CAS No. | 209799-67-7 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Forodesine 209799-67-7 Others 呋咯地辛 BCX 1777 BCX-1777 BCX1777 Immucillin-H Inhibitor inhibitor inhibit