Powder: -20°C for 3 years | In solvent: -80°C for 1 year
AMG 487 is an effective and selective antagonist of chemokine receptor 3. AMG 487 inhibited CXCR3-mediated cell migration induced by three CXCR3 chemokines, IP-10, ITAC and MIG (IC50: 8, 15 and 36 nM, respectively).
产品描述 | AMG 487 is an effective and selective antagonist of chemokine receptor 3. AMG 487 inhibited CXCR3-mediated cell migration induced by three CXCR3 chemokines, IP-10, ITAC and MIG (IC50: 8, 15 and 36 nM, respectively). |
体外活性 | AMG487 blocked in vitro lymphocyte migration mediated by CXCL9, CXCL10 or CXCL11. AMG487 did not affect the proliferation of 66.1 tumor cells at 24, 48 or 72 hours under normal or serum-free growth conditions. It did inhibit the migration of the tumor cells to CXCL9 by 70% [3]. |
体内活性 | AMG487 displayed an inhibitory effect on experimental lung metastasis. Treatment with bleomycin via intra-tracheal installation after tracheostomy induced cellular recruitment into the lungs in the lungs of mice. All but the lowest dose AMG 487 treatment group showed a significant reduction in cellular infiltration into the lungs (p < 0.005 as determined by Student’s t-test). Subcutaneously administration with AMG 487 (3 mg/kg, twice daily) showed migration inhibition results similar to those of CXCR3 deficient mice (n = 8-12 mice per group). The highly malignant murine mammary tumor cell line 66.1 was pretreated with AMG 487 and hence i.v. injected into immune-competent female mice [2][3]. |
分子量 | 603.59 |
分子式 | C32H28F3N5O4 |
CAS No. | T22477 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: <60.36mg/ml
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
(±)-AMG 487 T22477 Others AMG-487 (±)AMG 487 AMG487 AMG 487 (±) AMG 487 Inhibitor inhibitor inhibit