SARS-CoV-2 Nucleocapsid Protein (A220V, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 46.6 kDa and the accession number is P0DTC9.
生物活性 | Testing in progress |
产品描述 | SARS-CoV-2 Nucleocapsid Protein (A220V, His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 46.6 kDa and the accession number is P0DTC9. |
种属 | SARS-CoV-2 |
表达系统 | E. coli |
标签 | N-His |
蛋白编号 | P0DTC9 |
蛋白构建 | SARS-CoV-2 Nucleocapsid (YP_009724397.2, with mutation A220V) (Met1-Ala419) |
蛋白纯度 | > 90 % as determined by SDS-PAGE. |
分子量 | 46.6 kDa (predicted) |
内毒素 | Please contact us for more information. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM PB, 500 mM NaCl, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. The coronavirus N protein is required for coronavirus RNA synthesis and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is the most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to the formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of the N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
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recombinant recombinant-proteins proteins protein