PD-L1 Protein, Human, Recombinant (mFc)

PD-L1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 51.7 kDa and the accession number is Q9NZQ7-1.

规格	价格/CNY		货期	数量
100 μg	￥ 2,530	5日内发货
1 mg	￥ 16,500	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 2,530

5日内发货

1 mg

￥ 16,500

5日内发货

生物活性

1. Measured by its binding ability in a functional ELISA. Immobilized recombinant Human PD1-His at 10 μg/ml (100 μl/well) can bind human PD-L1 with a linear range of 1.28-20 μg/ml. 2. Labeled biotin to PD-L1 Protein, Human, Recombinant (ECD, Fc Tag) by a certain molar ratio;Using the Octet RED System, the affinity constant (Kd) of PD-L1 Protein, Human, Recombinant (ECD, Fc Tag), Biotinylated bound to Atezolizumab was 0.1 nM.

产品描述

PD-L1 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 51.7 kDa and the accession number is Q9NZQ7-1.

种属	Human
表达系统	HEK293 Cells
标签	C-mFc
蛋白编号	Q9NZQ7-1
别名	CD274 molecule, B7-H1, PDL1, PDCD1L1, B7H1, B7-H, PDCD1LG1, PD-L1
蛋白构建	The Human CD274 (NP_054862.1) (Met1-Thr239) was expressed with the Fc region of Mouse IgG1 at the C-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE.
分子量	51.7 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from 20 mM Tris, 150 mM NaCl, 10% Glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1/B7-H1 is a member of the growing B7 family of immune molecules and this protein contains one V-like and one C-like Ig domain within the extracellular domain, and together with PD-L2, are two ligands for PD1 which belongs to the CD28/CTLA4 family expressed on activated lymphoid cells. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Blockade: Blocking AntibodiImmune Checkpoint Blockade: PD-L1 / B7-H1 / CImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint Detection: IHC AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyPD-L1 / B7-H1 / CD274 Immune Checkpoint ProteTargeted Therapy