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NAA10/ARD1 Protein, Human, Recombinant (His & GST)

产品编号 TMPY-03339

别名: ARD1P, DXS707, ARD1, NATD, ARD1A, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, TE2, OGDNS, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit

NAA10/ARD1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 54 kDa and the accession number is P41227.

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NAA10/ARD1 Protein, Human, Recombinant (His & GST)

规格	价格/CNY		货期	数量
100 μg	￥ 3,820		5日内发货

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生物活性	Testing in progress
产品描述	NAA10/ARD1 Protein, Human, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 54 kDa and the accession number is P41227.

种属	Human
表达系统	Baculovirus Insect Cells
标签	His, GST
蛋白编号	P41227
别名	ARD1P, DXS707, ARD1, NATD, ARD1A, N(alpha)-acetyltransferase 10, NatA catalytic subunit, MCOPS1, TE2, OGDNS, NAA10, N(α)-acetyltransferase 10, NatA catalytic subunit
蛋白构建	The Human ARD1A (P41227) (Met1-Ser235) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
蛋白纯度	> 95 % as determined by SDS-PAGE
分子量	54 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	ARD1 is a member of the 2-kDa ARF protein family. It is a multifunctional protein. ARD1 has an 18-kDa ADP-ribosylation factor (ARF) domain at the C-terminus (amino acids 43-574), and a 46-kDa N-terminal domain (amino acids 1-42). The C-terminal region of ARD1 may be involved in the formation of both ARD1-ARD1 and ARD1-NAT1 complexes. ARD1 and NAT1 genes are required for the expression of an N-terminal protein acetyltransferase. This activity is required for full repression of the silent mating-type locus HML, for sporulation, and for entry into G. Recombinant ARD1 (amino acids 1-574) or its RING finger domain (amino acids 1-11) produced polyubiquitylated proteins when incubated in vitro with a mammalian E1, an E2 enzyme, ATP, and ubiquitin.

参考文献

1. Tribioli C.,et al.,(1994), Isolation of new genes in distal Xq28: transcriptional map and identification of a human homologue of the ARD1 N-acetyl transferase of Saccharomyces cerevisiae. Hum. Mol. Genet. 3:1061-1068. 2. Arnesen T.,et al., (2005), Identification and characterization of the human ARD1-NATH protein acetyltransferase complex.Biochem. J. 386:433-443. 3. Ross M.T.,et al.,(2005), The DNA sequence of the human X chromosome.Nature 434:325-337.

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Keywords

NAA10/ARD1 Protein, Human, Recombinant (His & GST) ARD1P DXS707 TE 2 ARD1 MCOPS 1 NATD ARD1A MCOPS-1 N(alpha)-acetyltransferase 10, NatA catalytic subunit ARD-1 MCOPS1 TE2 DXS 707 ARD 1 NAA 10 DXS-707 OGDNS NAA10 N(α)-acetyltransferase 10, NatA catalytic subunit NAA-10 TE-2 recombinant recombinant-proteins proteins protein

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