MICB Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 59.5 kDa and the accession number is A0A7D9H7X8.
生物活性 | Immobilized human His-NKG2D (78-216) at 10 μg/ml (100 μl/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15.9-37.1 ng/ml. |
产品描述 | MICB Protein, Human, Recombinant (His & hFc) is expressed in HEK293 mammalian cells with His and hFc tag. The predicted molecular weight is 59.5 kDa and the accession number is A0A7D9H7X8. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | His, hFc |
蛋白编号 | A0A7D9H7X8 |
别名 | MHC class I polypeptide-related sequence B, PERB11.2 |
蛋白构建 | The extracellular domain of Human MICB (NP_005922.2) (Met 1-Gly 298) was fused with the C-terminal polyhistidine-tagged Fc region of Human IgG1 at the C-terminus. |
蛋白纯度 | > 98 % as determined by SDS-PAGE |
分子量 | 59.5 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | MHC class I polypeptide-related sequence B, also known as MICB, is a heavily glycosylated protein serving as a ligand for the type II receptor NKG2D. MICB shares 85% amino acid identity with MICA, a closely related protein, both of which contain three extracellular immunoglobulin-like domains, but without the capacity to bind peptide or interact with beta-2-microglobulin. acting as a stress-induced self-antigen, binding of MICB to the NKG2D receptor activates the cytolytic response of natural killer (NK) cells, CD8+αβ T cells, and γδ T cells on which the receptor is expressed. MICA/B is minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B recognition thus is involved in tumor surveillance, viral infections, and autoimmune diseases. |
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MICB Protein, Human, Recombinant (His & hFc) MHC class I polypeptide-related sequence B PERB11.2 recombinant recombinant-proteins proteins protein