Mer Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 61.7 kDa and the accession number is Q60805.
生物活性 | The specific activity was determined to be 15 nmol/min/mg using Poly(Glu,Tyr) 4:1 as substrate |
产品描述 | Mer Protein, Mouse, Recombinant (His & GST) is expressed in Baculovirus insect cells with His and GST tag. The predicted molecular weight is 61.7 kDa and the accession number is Q60805. |
种属 | Mouse |
表达系统 | Baculovirus Insect Cells |
标签 | His, GST |
蛋白编号 | Q60805 |
别名 | Eyk, Mer, MER proto-oncogene, tyrosine kinase, Nyk, nmf12 |
蛋白构建 | The Mouse MERTK (Q60805) (Glu573-Tyr867) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
蛋白纯度 | > 96 % as determined by SDS-PAGE |
分子量 | 61.7 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10% gly. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. |
研究背景 | Proto-oncogene tyrosine-protein kinase MER (MERTK) is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. MERTK is localized in the membrane and is no expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. This protein is highly expressed in the testis, ovary, prostate, lung, and kidney, with lower expression in the spleen, small intestine, colon, and liver. MERTK regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization, and engulfment. MERTK plays also an important role in the inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces the production of suppressors of cytokine signaling SOCS1 and SOCS3. Defects in MERTK are the cause of retinitis pigmentosa type 38. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
Mer Protein, Mouse, Recombinant (His & GST) Eyk Mer MER proto-oncogene, tyrosine kinase Nyk nmf12 recombinant recombinant-proteins proteins protein