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Influenza A H3N2 (A/Sydney/5/1997) Hemagglutinin/HA Protein (His)

产品编号 TMPY-00026

别名: Harvey rat sarcoma viral oncogene homolog

Influenza A H3N2 (A/Sydney/5/1997) Hemagglutinin/HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.3 kDa and the accession number is C3PR59.

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Influenza A H3N2 (A/Sydney/5/1997) Hemagglutinin/HA Protein (His)

规格	价格/CNY		货期	数量
100 μg	￥ 4,460		5日内发货

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参考文献

生物活性	Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.03-0.15 μg/mL for 1% GRBC
产品描述	Influenza A H3N2 (A/Sydney/5/1997) Hemagglutinin/HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.3 kDa and the accession number is C3PR59.

种属	H3N2
表达系统	Baculovirus Insect Cells
标签	C-His
蛋白编号	C3PR59
别名	Harvey rat sarcoma viral oncogene homolog
蛋白构建	The Influenza A virus (A/Sydney/5/1997 (H3N2)) hemagglutinin (ACO95259.1) (Met1-Trp530), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
蛋白纯度	> 85 % as determined by SDS-PAGE.
分子量	59.3 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

参考文献

1. White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997. 2. Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005. 3. Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996. 4. Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018.

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Keywords

Influenza A H3N2 (A/Sydney/5/1997) Hemagglutinin/HA Protein (His) Harvey rat sarcoma viral oncogene homolog recombinant recombinant-proteins proteins protein

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