FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His)

FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 43.5 kDa and the accession number is Q8SPV9&Q8SPW0.

规格	价格/CNY		数量
100 μg	￥ 4,470	现货
200 μg	￥ 7,900	5日内发货
500 μg	￥ 16,710	5日内发货

规格

价格/CNY

货期

数量

100 μg

￥ 4,470

现货

200 μg

￥ 7,900

5日内发货

500 μg

￥ 16,710

5日内发货

生物活性

1. Measured by its ability to bind human IgG1 in functional ELISA.
2. Loaded Cynomolgus FcRn recombinant protein on His1K Biosensor, can bind IgG1 Fc with an affinity constant of 0.032 μM as determined in a BLI assay.
3. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.09 µM as determined in an SPR assay.
4. Captured Cynomolgus FcRn recombinant protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.18 µM as determined in an SPR assay (Biacore T200).
5. The purity of Cynomolgus FCGRT & B2M Heterodimer Protein is more than 95% and the molecular weight of this protein is around 40-60 kDa verified by SEC-MALS.

TargetMol Bioactive

产品描述

种属	Cynomolgus
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q8SPV9&Q8SPW0
别名	Fc fragment of IgG, receptor, transporter, alpha, Fc fragment of IgG, receptor, transporter, α
蛋白构建	The extracellular domain (Met 1-Ser 297) of cynomolgus FCGRT (Q8SPV9) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; The cynomolgus B2M (Q8SPW0) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified.
蛋白纯度	> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
分子量	43.5 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.

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Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

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FCGRT & B2M Heterodimer Protein, Cynomolgus, Recombinant (His)

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