CD16a Protein, Human, Recombinant (F176V, His & Avi), Biotinylated

CD16a Protein, Human, Recombinant (F176V, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.6 kDa and the accession number is P08637-1.

规格	价格/CNY		货期	数量
20 μg	￥ 2,940	5日内发货
100 μg	￥ 6,800	5日内发货

规格

价格/CNY

货期

数量

20 μg

￥ 2,940

5日内发货

100 μg

￥ 6,800

5日内发货

生物活性

1.Immobilized Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) at 10 μg/mL can bind recombinant human IgG1 (Fc) with a linear range of 0.31-5.0 μg/mL. 1.Immobilized Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) at 10 μg/mL can bind recombinant human IgG1 (Fc) with a linear range of 0.31-5.0 μg/mL. 2.Loaded Biotinylated Human FcγRIIIA / CD16a (V176) recombinant protein (His & Avi Tag) on SA Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 3.3 μM as determined in a BLI assay (Sartorius Octet Red384). 3.Loaded Biotinylated Human CD16a (V176) recombinant protein, His Tag on SA Biosensor, can bind Bevacizumab with an affinity constant of 0.74 μM as determined in BLI assay (Sartorius Octet Red384) (Routinely tested).

产品描述

种属	Human
表达系统	HEK293 Cells
标签	C-Avi-His
蛋白编号	P08637-1
别名	IMD20, FCRIIIA, FCG3, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), CD16, CD16A, FCRIII, Fc gamma RIIIa, FCGRIII, FCGR3, FCR-10, IGFR3, Fc γ RIIIa
蛋白构建	The extracellular domain (Met1-Gln208) of Human CD16a (P08637-1, with natural variant Phe 176 Val) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus (AVI-his). The expressed protein was biotinylated in vivo by the Biotin–Protein ligase (BirA enzyme) which is co-expressed.
蛋白纯度	> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC
分子量	25.6 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation.