CD16a Protein, Human, Recombinant (176F, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,820 | 5日内发货 | ||
1 mg | ¥ 24,900 | 5日内发货 |
生物活性 | 1.Loaded Human FcγRIIIA / CD16a (F176) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 1.9 μM as determined in a BLI assay. 2.Captured Human FcγRIIIA / CD16a (F176) recombinant protein on Anti-His Chip can bind Human IgG Fc with an affinity constant of 7.1 μM as determined in an SPR assay (Biacore T200). 3.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIIA / CD16a (F176) recombinant protein with an affinity constant of 2.67 µM as determined in an SPR assay. 4. The purity of Human CD16a / FCGR3A Protein (176 Phe, His Tag) is more than 95% and the molecular weight of this protein is around 36-48 kDa verified by SEC-MALS. |
产品描述 | CD16a Protein, Human, Recombinant (176F, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 23.3 kDa and the accession number is P08637-1. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-His |
蛋白编号 | P08637-1 |
别名 | FCR-10, CD16A, IGFR3, Fc gamma RIIIa, Fc fragment of IgG, low affinity IIIa, receptor (CD16a), FCGR3, FCG3, Fc γ RIIIa, FCRIII, CD16, FCRIIIA, IMD20, FCGRIII |
蛋白构建 | extracellular domain (Met 1-Gln 208) of Human CD16a (P08637-1) precursor |
蛋白纯度 | > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC |
分子量 | 23.3 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells. CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. |
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CD16a Protein, Human, Recombinant (176F, His) CD-16 FCR-10 FCGR-3 CD16A IGFR3 Fc gamma RIIIa Fc fragment of IgG, low affinity IIIa, receptor (CD16a) FCGR3 FCG3 FCR10 IMD 20 CD16a IGFR 3 CD 16 Fc γ RIIIa FCRIII IMD-20 FCGR 3 FCG 3 Fc fragment of IgG, low affinity IIIa, receptor Fc gamma RIIIa CD16 FCRIIIA IGFR-3 IMD20 FCG-3 FCR 10 FCGRIII recombinant recombinant-proteins proteins protein