CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.3 kDa and the accession number is A0A140HDP8.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,820 | 5日内发货 | ||
1 mg | ¥ 24,900 | 5日内发货 |
生物活性 | 1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD16-AVI-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.2-0.5 μg/ml. 2. Labeled biotin to CD16 / FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD16 / FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag), Biotinylated bound to IgG1 Antibody was 0.1 μM. |
产品描述 | CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.3 kDa and the accession number is A0A140HDP8. |
种属 | Rhesus |
表达系统 | HEK293 Cells |
标签 | His, Avi |
蛋白编号 | A0A140HDP8 |
别名 | Fc receptor, IgG, low affinity III |
蛋白构建 | The rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. |
蛋白纯度 | > 95 % as determined by SDS-PAGE |
分子量 | 25.3 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes. |
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CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi) Fc receptor, IgG, low affinity III recombinant recombinant-proteins proteins protein