store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Tetrahydrobiopterin (BH4) 是芳香族氨基酸羟化酶的辅因子, 也是一氧化氮合酶 (NOS) 的必需辅因子,可用于研究如高血压、高胆固醇血症、糖尿病等内皮功能障碍。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
2 mg | ¥ 664 | 现货 | ||
5 mg | ¥ 1,100 | 现货 | ||
1 mL * 10 mM (in DMSO) | ¥ 1,200 | 现货 |
产品描述 | Tetrahydrobiopterin (BH4) is a cofactor for aromatic amino acid hydroxylases and an essential cofactor for nitric oxide synthase (NOS), which is used in the study of endothelial dysfunction such as hypertension, hypercholesterolemia, and diabetes. |
体外活性 | Microglia cultures under hyperoxic conditions were supplemented with or without Tetrahydrobiopterin (100 μM). Exposure of microglia to hyperoxia-induced oxidative stress for 24 h showed a significant increase in TSP-1 mRNA expression and protein compared with normoxia (21% O2). Tetrahydrobiopterin supplementation significantly prevents hyperoxia-induced microglial activation and prevents microvascular damage in choroidal explants by reducing Iba-1 and TSP-1 expression.[1] |
体内活性 | To assess the levels of tetrahydrobiopterin in the retina, three to five retinal pools were collected from WT and HPH-1 mice at postnatal ages 7, 14, and 22 years and evaluated by LC-MS/MS. LC-MS/MS analysis confirmed that the concentration level of tetrahydrobiopterin in the retinal tissue of HPH-1 mice was significantly reduced by approximately 90% compared with WT groups P7, P14, and P22, respectively.[1] |
别名 | Sapropterin, BH4, (Rac)-Sapropterin |
分子量 | 241.25 |
分子式 | C9H15N5O3 |
CAS No. | 17528-72-2 |
store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 40 mg/mL (165.80 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.1451 mL | 20.7254 mL | 41.4508 mL | 103.6269 mL |
5 mM | 0.829 mL | 4.1451 mL | 8.2902 mL | 20.7254 mL | |
10 mM | 0.4145 mL | 2.0725 mL | 4.1451 mL | 10.3627 mL | |
20 mM | 0.2073 mL | 1.0363 mL | 2.0725 mL | 5.1813 mL | |
50 mM | 0.0829 mL | 0.4145 mL | 0.829 mL | 2.0725 mL | |
100 mM | 0.0415 mL | 0.2073 mL | 0.4145 mL | 1.0363 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Tetrahydrobiopterin 17528-72-2 Immunology/Inflammation Metabolism NOS Endogenous Metabolite BH 4 Sapropterin BH4 (Rac)-Sapropterin BH-4 Inhibitor inhibitor inhibit