keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Sulforhodamine B sodium salt (Acid Red 52) 是一种能够定量测定培养细胞的细胞蛋白量的荧光染料,。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
50 mg | ¥ 298 | 现货 | ||
100 mg | ¥ 418 | 现货 | ||
1 g | ¥ 587 | 现货 |
产品描述 | Sulforhodamine B sodium salt (Acid Red 52) is a fluorescent dye. It can be used from laser-induced fluorescence to the quantification of cellular proteins of cultured cells. |
体外活性 | Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via the determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell-based studies. The protocol can be divided into four main steps: preparation of treatment, incubation of cells with treatment of choice, cell fixation, and SRB staining, and absorbance measurement. This assay is limited to manual or semiautomatic screening and can be used in an efficient and sensitive manner to test chemotherapeutic drugs or small molecules in adherent cells. It also has applications in evaluating the effects of gene expression modulation (knockdown, gene expression upregulation), as well as to study the effects of miRNA replacement on cell proliferation. |
别名 | Acid Red 52, Kiton Red 620, 磺酰罗丹明B |
分子量 | 580.65 |
分子式 | C27H29N2NaO7S2 |
CAS No. | 3520-42-1 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (86.11 mM), Sonication is recommended.
H2O: 20 mg/mL (34.44 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO / H2O | 1 mM | 1.7222 mL | 8.611 mL | 17.2221 mL | 43.0552 mL |
5 mM | 0.3444 mL | 1.7222 mL | 3.4444 mL | 8.611 mL | |
10 mM | 0.1722 mL | 0.8611 mL | 1.7222 mL | 4.3055 mL | |
20 mM | 0.0861 mL | 0.4306 mL | 0.8611 mL | 2.1528 mL | |
DMSO | 50 mM | 0.0344 mL | 0.1722 mL | 0.3444 mL | 0.8611 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Sulforhodamine B sodium salt 3520-42-1 Others Inhibitor Sulforhodamine B Acid Red52 Acid Red 52 Kiton Red-620 Kiton Red 620 inhibit 磺酰罗丹明B Kiton Red620 Sulforhodamine B sodium Acid Red-52 inhibitor