keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Procion Blue HB is an antagonist of purinergic.
产品描述 | Procion Blue HB is an antagonist of purinergic. |
体外活性 | Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+ oscillations in HeLa cells. Reactive Blue 2 enhances a Ca2+ response to histamine that is linked to the PLC cascade. Reactive Blue 2 may activate the PLC cascade in an extracellular Ca2+-dependent manner and induce Ca2+ oscillations [1]. The application of Reactive Blue 2 increases K+ secretion in a dose-dependent manner. Reactive Blue 2 has antagonistic activities at P2Y4, and the antagonist potency at P2Y4 paralleled the potency of K+ secretion [2]. The anthraquinone dye reactive blue 2 is found to be a potent inhibitor of a protein kinase isolated and purified from thylakoids. The mode of inhibition is noncompetitive, with a Ki of 8 μM for the membrane-bound kinase, and 6 μM for the purified kinase. The inhibitor does not modify the substrate preference of the endogenous kinase and could be removed from the membrane by washing[3]. Reactive Blue 2 selectively inhibits responses mediated via the P2ypurinoceptor, at least within a limited concentration range. In preparations where the tone has been raised with ATP, noradrenaline, and 2-methylthio ATP, but not α,β-methylene ATP, produces relaxations of the vessel. These relaxations are inhibited in the presence of reactive blue 2 [4]. Reactive blue 2 (0.3-10 μM) blocks the ATP-induced oscillation in a concentration-dependent manner [5]. |
别名 | 蓝色染料, Reactive Blue 2 |
分子量 | 1548.29 |
分子式 | C58H40Cl2N14O22S6 |
CAS No. | 12236-82-7 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 10 mg/mL (12.92 mM), Sonication is recommended.
DMSO: 110 mg/mL (142.09 mM)
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
H2O / DMSO | 1 mM | 0.6459 mL | 3.2294 mL | 6.4587 mL | 16.1468 mL |
5 mM | 0.1292 mL | 0.6459 mL | 1.2917 mL | 3.2294 mL | |
10 mM | 0.0646 mL | 0.3229 mL | 0.6459 mL | 1.6147 mL | |
DMSO | 20 mM | 0.0323 mL | 0.1615 mL | 0.3229 mL | 0.8073 mL |
50 mM | 0.0129 mL | 0.0646 mL | 0.1292 mL | 0.3229 mL | |
100 mM | 0.0065 mL | 0.0323 mL | 0.0646 mL | 0.1615 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Procion Blue HB 12236-82-7 Others 蓝色染料 Reactive Blue 2 Inhibitor inhibitor inhibit