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Nile Blue A sulfate

产品编号 T19034 CAS 3625-57-8

别名: Nile blue sulfate

Nile Blue A (Nile blue sulfate) is used as the differentiation of melanins and lipofuscins. It is also useful for staining fats and the preparation of an amperometric glucose sensor.

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Nile Blue A sulfate, CAS 3625-57-8

规格	价格/CNY		货期	数量
500 mg	￥ 995		待询

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生物活性

化学信息

存储 & 溶解度

参考文献

产品描述

Nile Blue A (Nile blue sulfate) is used as the differentiation of melanins and lipofuscins. It is also useful for staining fats and the preparation of an amperometric glucose sensor.

体外活性

Nile blue A is a satisfactory stain for PHB granules in bacteria and is in fact superior to Sudan black B for this purpose. Poly-p3-hydroxybutyrate granules exhibit a strong orange fluorescence when stained with Nile blue A. Nile blue A appears to stain many more PHB granules than Sudan black B does and is not as easily ished from the cell by decolorization procedures [1]. Nile blue A is used as a stain for polyhydroxyalkanoic acid-accumulating microorganisms or to detect polyhydroxyalkanoic acids in microorganisms. Escherichia coli cells that do not accumulate detectable polyhydroxyalkanoic acids can be stained with Nile blue A. Nile blue A staining does not affect either surface display of peptides or specific labeling of these peptides by a second fluorescence. Staining E. coli for flow cytometry using Nile blue A is an easy-to-handle and low-cost alternative to other fluorescent dyes or the intracellular expression of, for example, green fluorescent protein [2]. Nile blue A is used as a potent photosensitizer for photodynamic therapy. The dye when administered intravenously disperses throughout the body by circulating through blood and is taken up by most cells that emphasize its interaction with various biomolecule [3].

别名	Nile blue sulfate
分子量	414.46
分子式	C20H20N3O5S
CAS No.	3625-57-8

存储

keep away from direct sunlight　|　Powder: -20°C for 3 years | In solvent: -80°C for 1 year

溶解度

DMSO: 150 mg/mL (409.37 mM)

溶液配制表

可选溶剂	浓度体积质量	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4128 mL	12.0639 mL	24.1278 mL	60.3195 mL
	5 mM	0.4826 mL	2.4128 mL	4.8256 mL	12.0639 mL
	10 mM	0.2413 mL	1.2064 mL	2.4128 mL	6.0319 mL
	20 mM	0.1206 mL	0.6032 mL	1.2064 mL	3.016 mL
	50 mM	0.0483 mL	0.2413 mL	0.4826 mL	1.2064 mL
	100 mM	0.0241 mL	0.1206 mL	0.2413 mL	0.6032 mL

计算器

摩尔浓度计算器

稀释计算器

配液计算器

分子量计算器

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参考文献

1. Ostle AG, et al. Nile blue A as a fluorescent stain for poly-beta-hydroxybutyrate. Appl Environ Microbiol. 1982 Jul;44(1):238-41. 2. Betscheider D, et al. Nile blue A for staining Escherichia coli in flow cytometer experiments. Anal Biochem. 2009 Jan 1;384(1):194-6. 3. Mishra SS, et al. Spectroscopic investigation of interaction of Nile Blue A, a potent photosensitizer, with bile salts in aqueous medium. J Photochem Photobiol B. 2014 Dec;141:67-75.

剂量换算

对于不同动物的给药剂量换算，您也可以参考 更多...

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：比如您的给药剂量是10 mg/kg，每只动物体重20 g，给药体积100 μL，一共给药动物10 只，您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。

母液配置方法：2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL)，如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法：取 50 μL DMSO 主液，加入 300 μL PEG300，混匀澄清，再加 50 μL Tween 80，混匀澄清，再加 600 μL ddH2O, 混匀澄清。

第一步：请输入动物实验的基本信息

剂量

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μL

动物数量

第二步：请输入动物体内配方组成，不同的产品配方组成不同，如有配方需求，可先联系我们提供正确的体内配方。

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