Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Kinesore is a KLC2-SKIP Interaction inhibitor.
产品描述 | Kinesore is a KLC2-SKIP Interaction inhibitor. |
体外活性 | The microtubule network is entirely reorganized into a series of loops and bundles, in kinesore-treated cells. The lysosomal compartment accumulates in a juxtanuclear position, where there are relatively few microtubules. At 50 μM kinesore, this phenotype is highly penetrant, with 95±2.4% (n=3, total of 200 cells) of cells exhibiting a reorganized nonradial microtubule network. In wild-type cells, 50 μM kinesore induces the remodeling of the microtubule network and the formation of extensive microtubule-rich projections. This phenotype is strongly suppressed in Kif5B knockout cells, confirming that microtubule remodeling induced by kinesore is dependent upon the presence of kinesin-1. In titration experiments, in cells treated for 1 h, this phenotype becomes apparent at a concentration of 25 μM kinesore, with relatively little effect at or below concentrations of 12.5 μM. The effect is reversible because a 2-h washout of kinesore from cells treated for 1 h led to the reestablishment of the radial microtubule array. This kinesore-induced reorganization of the microtubule network is observed in a panel of mammalian normal and cancer cell lines[1]. |
分子量 | 536.17 |
分子式 | C20H16Br2N4O4 |
CAS No. | 363571-83-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 125 mg/mL (233.14 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 1.8651 mL | 9.3254 mL | 18.6508 mL | 46.627 mL |
5 mM | 0.373 mL | 1.8651 mL | 3.7302 mL | 9.3254 mL | |
10 mM | 0.1865 mL | 0.9325 mL | 1.8651 mL | 4.6627 mL | |
20 mM | 0.0933 mL | 0.4663 mL | 0.9325 mL | 2.3314 mL | |
50 mM | 0.0373 mL | 0.1865 mL | 0.373 mL | 0.9325 mL | |
100 mM | 0.0187 mL | 0.0933 mL | 0.1865 mL | 0.4663 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Kinesore 363571-83-9 Others Inhibitor inhibitor inhibit